Purification of biotechnological xylitol from Candida tropicalis fermentation using activated carbon in fixed-bed adsorption columns with continuous feed

Xylitol, a sugar substitute, presents considerable amount of benefits for both product applications and consumer health, which make it high value various industries. The production xylitol through yeast fermentation is an alternative that suits sustainable development, as can use agro-industrial byproducts, might come to reach lower price than the current method in once its technology further developed. While there extensive research process up synthesis xylitol, purification attain final still not well consolidated. In present work, tests were performed assess capacity containing fermented media by activated carbon fixed bed columns. Response surface methodology with pulse input was utilized investigate how responses vary when temperature, superficial velocity injection volume are altered, define conditions maximize productivity minimizes retention while maintaining satisfactory levels clarification. best condition found (T = 70 °C, vs 1.5 cm/min, Vi 3 mL) then applied new test continuous feeding media. presented potential biotechnological step efficacy separating proteins (PFprotein 18.0), colored compounds (reducing more 99% absorbance at 420 nm 560 nm) ethanol (PFethanol 20.4) 36.8 g L−1 h−1 RCxylitol 31.1%.